Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. JNK1_Mw; 48,000 JNK3_Mw; 53,000
HEK293 細胞を,Ser/Thr phosphatase 阻害剤のcalyculin A,またはTyr phosphatase 阻害剤のpervanadateで処理した後,JNK1/3のリン酸化状態の変化を Zn2+-Phos-tag SDS-PAGEで調べています。
c-Jun
SAPK/JNK
Zn2+ -Phos-tag: 25µM
Polyacrylamide: 8.5%(w/v)